α-MEM,nucleosides,GlutaPlus, 500 mL

Attribute:

SKU : G4554-500ML

Brand : Servicebio

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Product Information

Product Name

Cat. No.

Spec.

α-MEM, nucleosides, GlutaPlus

G4554-500ML

500 mL

 


Product Description/Introduction

α-MEM (α Minimum Essential Medium) is a modified medium from MEM. Compared with MEM , α-MEM has added sodium pyruvate, zinc sulfate, vitamin B12, biotin, ascorbic acid and other components, and is widely used for the culture of various mammalian suspension and adherent cells. α-MEM is divided into the types of nucleoside-containing and non-nucleoside-containing. The nucleoside-free and deoxynucleoside -free α-MEM is commonly used as a screening medium for DG44 and other DHFR-deficient cells.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add serum or serum-free additives depending on the cell type.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains L-Alanyl-L-Glutamine, sodium pyruvate, phenol red indicator, nucleoside and deoxynucleoside, without HEPES. Please check the official website for the specific recipe.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

For Research Use Only!

G4554 α-MEM Formula

Components

Concentration


Components

Concentration

mg/L

mM

mg/L

mM

Amino acids




Inorganic salts



Glycine

50.0

0.6667


Calcium Chloride (CaCl2)(anhyd.)

200

1.801802

L-Alanine

25.0

0.2809


Magnesium Sulfate (MgSO4) (anhyd.)

97.67

0.813917

L-Arginine hydrochloride

105.0

0.4976


Potassium Chloride (KCl)

400

5.333

L-Asparagine-H2O

50.0

0.3333


Sodium Bicarbonate (NaHCO3)

2200

26.2

L-Aspartic Acid

30.0

0.2256


Sodium Chloride (NaCl)

6800

117.2

L-Cystine 2HCl

31.0

0.0990


Sodium Phosphate monobasic (NaH2PO4) (anhyd.)

121.7

1.014

L-Cysteine hydrochloride-H2O

100.0

0.5682


Other components



L-Glutamic Acid

75.0

0.5102


D-Glucose (Dextrose)

1000

5.6

L-Alanyl-L-Glutamine

434.4

2.0


Lipoic Acid

0.20

0.0009709

L-Histidine hydrochloride-H2O

42

0.20


Phenol Red

9.40

0.026567

L-Isoleucine

52.4

0.40


Sodium pyruvate

110

1.0

L-Leucine

52.0

0.3969


Adenosine

10.00

0.375

L-Lysine hydrochloride

73.0

0.3989


Cytidine

10.00

0.0412

L-Methionine

15.0

0.1007


Guanosine

10.00

0.0353

L-Phenylalanine

32.0

0.1939


Uridine

10.00

0.0410

L-Proline

40.0

0.3478


2Deoxyadenosine

10.72

0.0398

L-Serine

25.0

0.2381


2Deoxycytidine HCl

9.46

0.0417

L-Threonine

48.0

0.4034


2Deoxyguanosine

10.68

0.0375

L-Tryptophan

10.0

0.0490


Thymidine

10.00

0.0413

L-Tyrosine Disodium Salt

52.0

0.2311





L-Valine

46.0

0.3932





Vitamins







Ascorbic Acid

50.0

0.2841





Biotin

0.10

0.000410





Choline chloride

1.0

0.007143





D-Calcium pantothenate

1.0

0.002096





Folic Acid

1.0

0.002268





Niacinamide

1.0

0.008197





Pyridoxine hydrochloride

1.0

0.004902





Riboflavin

0.10

0.000266





Thiamine hydrochloride

1.0

0.002967





Vitamin B12

1.36

0.001004





i-Inositol

2.0

0.011111





 

 

 

 

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