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Nucleic Acid Extraction│Servicebio
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Categories : 1. Chemical and Reagents ,  Servicebio , 
Brand : Servicebio
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Substitute for Chloroform (RNA Extraction Assisted Reagent)
Safe and low-toxic, it can completely replace chloroform during nucleic acid extraction.
Product Information
Product Name | Cat.No. | Spec. | Notes |
Substitute for Chloroform (RNA Extraction Assisted Reagent) | G3014-01 | 25 mL | This low-toxicity chloroform substitute can completely replace chloroform for RNA or DNA extraction, without affecting the quality and yield of nucleic acid extraction. |
Substitute for Chloroform (RNA Extraction Assisted Reagent) | G3014-02 | 100 mL | This low-toxicity chloroform substitute can completely replace chloroform for RNA or DNA extraction, without affecting the quality and yield of nucleic acid extraction. |
Description/Introduction
The effective component of this product is 1-Bromo-3-chloropropane, which is less toxic and is a substitute for chloroform. Because the reagent has stronger hydrophobicity, it can completely replace chloroform in RNA or DNA extraction by reagent method, and form a tight intermediate layer, which can reduce the total amount of the reagent, and has no effect on the quality and yield of separated RNA or DNA.
Storage and Shipping Conditions
Transported at room temperature and stored at room temperature; valid for 36 months.
Assay Protocol / Procedures
Extraction of RNA by reagent method as an example: when using 1 mL RNA Isolation Reagent (Cat. #: G3013) or TRIzol for single-step method extraction of RNA, replace 200 μL chloroform with 100 μL of the reagent, and the remaining steps remain unchanged.
Note
1. Please wear a lab coat when using this product, and wear gloves, masks, masks, etc.
2. The product is volatile, please use it in the fume hood. Tighten the bottle cap in time after use.