Sulfonamides ELISA Test Kit, Veterinary Drugs, 0.5 ppb
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SKU : ME08BER
Categories : 9. Food Safety , Meat & Seafood & Egg , ELISA , Meizheng (China) ,
Brand : MEIZHENG (China)
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Sulfonamides ELISA Test Kit for Poultry Tissues, Aquatic Tissue and Serum
The Sulfonamides ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of sulfonamides in samples of poultry tissues, aquatic tissue, serum, etc.
Description
Summary
Sulfonamides are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human.
They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their
effectiveness against gram-negative and gram-positive organisms.
The detection method is sensitive, fast, simple, and specific, only need 45mins for operating and a small amount of sampling.
Use Principles
The Sulfonamides ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen
compete the antibody (antibody solution) with sulfonamides in the sample, anti-sulfonamides antibody binds to the HRP enzyme conjugate. Then
pipette the substrate solution to the wells to convert the color. The color of unknown samples is compared to the color of the standards and the
sulfonamides concentration of the samples is derived.
SENSITIVITY (DETECTION LIMIT)
—- Sensitivity: 0.5ppb
Limit of detection
Tissue sample ……………………………………………………………………………….. 4ppb
Serum sample……………………………………………………………………………….. 5ppb
Cross-reactivity
Sulfadiazine …………………………………………………………………………………..100%
Sulfadoxine ……………………………………………………………………………………181%
Sulfamonomethoxine……………………………………………………………………..150%
Phthalylsulfathiazole (PST)……………………………………………………………….90%
Sulfamethoxydiazine………………………………………………………………………..85%
Sulfadimethoxypyrimidine ……………………………………………………………….67%
Benzene Sulfapyridine ……………………………………………………………………..62%
Sulfapyridine……………………………………………………………………………………55%
Sulfamerazine………………………………………………………………………………….52%
Sulfamethizole…………………………………………………………………………………49%
Sulfachlorpyridazine ……………………………………………………………………..29.6%
Sulfamethazine………………………………………………………………………………..13%
Percent Recovery, %
Tissue sample ………………………………………………………………………….. 80±10%
Serum sample………………………………………………………………………….. 90±15%
Precision (CV %):
Intra-lab assay: CV%≤10%
| Kit Contents | Quantity | Storage |
| Antigen coated plate | 96T | 2-8°C |
| 5 vials of Standard | 1mL each | 2-8°C |
| High concentration standard (1ppm) | 1mL | 2-8°C |
| Enzyme Conjugate | 6mL | 2-8°C |
| Antibody Solution | 9mL | 2-8°C |
| Substrate A | 6mL | 2-8°C |
| Substrate B | 6mL | 2-8°C |
| Concentrated Sample Diluting Buffer I (10× ) | 45mL | 2-8°C |
| Concentrated Sample Diluting Buffer II (2× ) | 45mL | - |
| Concentrated Wash Buffer (10× ) | 40mL | - |
| Package Insert | 1 | - |
| Testing Report | 1 | - |
| Plate Cover Sheet | 2 | - |
