Ofloxacin ELISA Test Kit, Veterinary Drugs, 0.06 ppb

Animal Tissue Antibiotic Ofloxacin ELISA Test Kit

Qualitative or quantitative analysis of Ofloxacin in animal tissue (pork,chicken, duck, fish, shrimp, chicken heart, chicken liver, chicken, gizzard).

Description

Order No.: ME11CER

LOD: 0.06 ppb

Package: 96T

[Summary]

Ofloxacin (OFL) is a synthetic third-generation quinolone antibacterial

drug with broad-spectrum antibacterial activity and good bactericidal

effect. It has antibacterial effect on Enterobacter, Pseudomonas

aeruginosa, Haemophilus influenzae, Neisseria gonorrhoeae,

Streptococcus, Legionella, and Staphylococcus aureus.

The Ofloxacin ELISA Test Kit produced by Meizheng is fast,

easy-to-use, accurate and has high sensitivity, with 45 minutes

operation time, which can minimize the operating error and working

intensity.

[Principles]

Ofloxacin ELISA Kit is an indirect competitive enzyme-labeled

immunoassay. The Ofloxacin antigen is precoated on the wells. The

precoated antigen compete the Ofloxacin antibody (Antibody Solution)

with Ofloxacin in the sample, Ofloxacin antibody binds to the

Ofloxacin Enzyme Conjugate. After the color development of TMB

Substrate, the absorbance value of the sample is negatively correlated

with the Ofloxacin contained in it. Compared with the standard curve

and multiplied by its corresponding dilution factor, the content of

Ofloxacin in the sample can be calculated.

[Cross Reactivity]

Ofloxacin (OFL) ······················································100%

Ciprofloxacin (CIP) ··················································· <1%

Enrofloxacin (ENR) ·····················································2%

Norfloxacin (NOR) ····················································<1%

Salafloxacin (SAR) ····················································<1%

Pefloxacin (PEF) ························································ 3%

Lomefloxacin (LOM) ················································· <1%

Danfloxacin (DAN) ··················································· <1%

Enoxacin (ENO) ······················································· <1%

Mapofloxacin (MAR) ················································130%

Difloxacin (DIF) ······················································· 28%

Flumequine ·····························································<1%

Nafloxacin ······························································<1%

Oxalic Acid ·····························································<1%

Obifloxacin ·····························································<1%

 [Materials Required But Not Provided]

1. Devices:
   • Microplate Reader (450nm/630nm filter)
     
   • Vortex
     
   • Constant Temperature Incubator (Optional)
     
   • Centrifuge
     
   • Homogenizer
     
   • Balance (sensibility: 0.01g)
     
   • Rubber Bulb Syringe
     
   • Termovap Sample Concentrator
     
   • Centrifuge tube: 5mL/7mL, 50mL
     
   • Graduated Pipette: 10mL
     
   • Micropipette: Single channel pipette (10-100µL, 100-1000µL)
     
   • Multi-channel pipette (30-300µL)
2. Reagent:
   • Deionized Water
     
   • Acetonitrile (AR)
     
   • N-hexane (AR)
     
   • Concentrated Hydrochloric Acid (HCl) (AR)
     
   • 18M Concentrated Sulfuric Acid (H2SO4)

[Provided Materials and Reagent]

Note: The concentrations of the standards are 0ppb, 0.06ppb, 0.18ppb, 0.54ppb,1.62ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

https://mzfoodtest.com/product/animal-tissue-antibiotic-ofloxacin-elisa-test-kit/