Amantadine ELISA Test Kit, Veterinary Drugs, 0.5 ppb

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SKU : ME24BER

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Amantadine ELISA Test Kit for Chicken and Pork

Description

【Summary】

Amantadine is a drug with multiple uses and varied mechanisms of action. Chinese poultry farmers used amantadine to protect birds against avian influenza, that cause avian flu (H5N1) strains in China and southeast Asia are now resistant to amantadine. However, in Western countries and according to international livestock regulations, amantadine is approved only for use in humans.

【Principles】

The Amantadine ELISA Test kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with Amantadine in the sample, anti-Amantadine antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Amantadine concentration of the samples is derived. 

【Scope of Application】

The Amantadine ELISA Test Kit is competitive ELISA for the qualitative or quantitative analysis of Amantadine in samples of chicken and pork.

【Cross-reactivity】 

Amantadine 100%

【Materials Required But Not Provided】

  1. Devices:
    • Microplate Reader (450nm/630nm filter)
    • Vortex
    • Rubber Bulb Syringe
    • Homogenizer
    • Centrifuge
    • Termovap Sample Concentrator
    • Constant temperature incubator (Optional)
    • Centrifuge tube (2mL, 10mL/7mL, 50mL)
    • Balance (sensibility: 0.01g)
    • Graduated pipette: 10mL
    • Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)
  2. Reagent:
    • Deionized water
    • Sodium Chloride (NaCl) (AR)
    • Acetonitrile (AR)
    • 18M concentrated sulfuric acid (H2SO4)

 

【Provided Materials and Reagent】

Component96 wells
Antigen coated plate96 T
6 vials of Standard1mL each
High concentration standard (1ppm)1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated sample diluting buffer40mL
Package Insert1
Testing Report1
Plate Cover Sheet2

 

Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb, 40.5ppb, respectively.

 Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

 

https://mzfoodtest.com/product/amantadine-elisa-test-kit-for-chicken-and-pork/

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