Lincomycin ELISA Test Kit, Veterinary Drugs, 0.2 ppb
Attribute:
SKU : ME35AER
Categories : 9. Food Safety , Meat & Seafood & Egg , ELISA , Meizheng (China) ,
Brand : MEIZHENG (China)
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Poultry and Seafood Antibiotic Lincomycin ELISA Test Kit
Qualitative or quantitative analysis of Lincomycin in tissue of poultry and seafood.
Description
Summary
Lincomycin is a Lincosamides antibiotic mainly used to restrain synthesis of bacterial protein. Lincomycin residual in livestock and poultry will hurt liver and cause allergic reaction and Cardiovascular reaction.
Order No.: ME35AER
LOD: 0.2 ppb
Package: 96T
Sample: Meat, Seafood
Use Principles
Lincomycin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Lincomycin antigen is precoated on the wells. The precoated antigen compete the Lincomycin antibody (antibody solution) with Lincomycin in the sample, anti- Lincomycin antibody binds to the Lincomycin -HRP enzyme conjugate. Then pipete the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Lincomycin concentration of the samples is derived.
Cross-reactivity
Lincomycin ………………………………………………100%
Clindamycin ………………………………………………＜1%
Spiramycin…………………………………………………＜1%
Erythromycin………………………………………………＜1%
Tylosin…………………………………………………………＜1%
Streptomycin………………………………………………＜1%
Kanamycin……………………………………………………＜1%
Spectinomycin………………………………………………＜1%
Materials Required But Not Provided
- Devices:
- Microwell plate Reader (450nm/630nm)
- Homogenizer
- Oscillator
- Vortex
- Centrifuge
- Balance (sensibility: 0.01g)
- Transfer pipette: 10mL
- Aurilave
- Centrifuge tube: 5mL, 7mL, 50mL
- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
Multi-channel pipet (30µL -300µL)
- Microwell plate Reader (450nm/630nm)
- Reagent:
- Deionized water
- Concentrated sulfuric acid (18 mol/L)
- Deionized water
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 6 vials of standard | 1mL each |
| High concentration standard (2ppm) | 1mL each |
| Enzyme Conjugate | 6mL |
| Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated Wash Buffer (10×) | 40mL |
| Concentrated sample diluting buffer (2×) | 50mL |
| Package Insert | 1 |
| Testing Report | 1 |
| Plate Cover Sheet | 2 |
Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb and 16.2ppb.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
https://mzfoodtest.com/product/poultry-and-seafood-antibiotic-lincomycin-elisa-test-kit/
