EMBER500™ RNA Prestain Loading Dye, 250 ul

Sensitive and easy-to-use RNA prestain loading dye allows RNA to be evaluated on a regular agarose gel.

PRODUCT DESCRIPTION
EMBER500™ RNA Prestain Loading Dye is a convenient reagent for single-step denaturing, staining, and loading of RNA for gel analysis. The loading dye contains EMBER500™, a bright and versatile fluorescent DNA and RNA binding dye with broad instrument compatibility.

• Fast, simple, and super-bright fluorescent RNA prestain
  
• Denaturing loading dye allows RNA to be run on a regular agarose gel
  
• Evaluate total RNA integrity and DNA contamination
  
• Far more sensitive than EtBr prestaining
  
• Compatible with UV or blue LED gel imagers and commonly used filters

For a limited time, Biotium is offering free samples of EMBER500™. The free sample is for U.S customers only. Click “Get Free Sample” below and fill out the form. Be sure to select EMBER500™ RNA Prestain Loading Dye 100 uL (#41032-F) under “Select a Sample” before submitting.

EMBER500™ prestaining gives much brighter signal and higher sensitivity than ethidium bromide prestaining. The dye stains RNA and DNA, allowing the detection of contaminating genomic DNA in purified RNA samples. The prestaining procedure is simpler, faster, and more sensitive than post-electrophoresis gel staining, and allows the use of a native agarose gel instead of a complicated and hazardous denaturing gel. EMBER500™ staining can be imaged using a UV transilluminator with standard filters, and also is compatible with blue LED gel imagers, which eliminate UV exposure hazards.

The loading dye is supplied at 2X concentration. It contains formamide for denaturing RNA, and the electrophoresis tracking dyes bromophenol blue and xylene cyanol, which migrate in agarose gels at ~300 bp and ~3 kb respectively.

Note that EMBER500™ prestaining is not recommended for analyzing low molecular weight RNA or ssDNA, or for staining denaturing gels such as formaldehyde, glyoxal, or urea gels. For these applications, we recommend performing post-staining with Oxazole Gold.

Human total cellular RNA in water was mixed with EMBER500™ RNA Prestain Loading Dye or loading dye with 250 ug ethidium bromide (EtBr). Samples were heated for 10 minutes at 70°C, then run on a non-denaturing 1% agarose/1X TBE gel. The gel was imaged using a UV transilluminator with EtBr filter.

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