nPfu-Forte (High Fidelity PCR), (2.5 Units/ul), 250U
Attribute:
SKU : P425
Categories : PCR Enzyme / RT-PCR , 2. Cell & Molecular Biology , Real-Time PCR , Enzynomics (Korea) ,
Brand : Enzynomics (South Korea)
Share
Product description
nPfu-Forte is Pfu DNA Polymerase supplemented with an enhancer, which maintains the activity of Pfu DNA Polymerase in the presence of inhibitors produced during high-temperature PCR cycles. Thus, high-fidelity DNA polymerization is obtained with nPfu-Forte, allowing amplification of DNA up to 10 kb long. nPfu-Forte is more effective than nPfu and has a high amplification rate (1 kb/min). Higher levels of PCR products are often obtained even with lowabundance template DNA.
Characteristics
- Molecular weight: 90 kDa
- Error rate: 2.8 X 10-7
- Thermal stability: Half life of 4 hr at 95℃
- Blunt end products
Applications
- High fidelity DNA amplification for cloning
- Amplification of long DNA fragments (<10 kb)
- DNA amplification to generate blunt end products
- Site-directed mutagenesis
- DNA sequencing
Supplied with
- 10X nPfu-Forte buffer
- dNTP Mixture (2 mM each)
- Sterile water
Quality control
- Purity: >99% on SDS-PAGE
- Endonuclease-free
- Exonuclease-free
- RNase-free
- Inhibitor-free
Unit definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid insoluble materials in 30min at 74℃ in a 50-μl reaction mixture (20 mM Tris-HCl/pH 8.8, 50 mM KCl, 2.5 mM MgCl2, 10 mM β-mercaptoethanol, 12.5 μg of calf thymus DNA).
10X nPfu-Forte buffer
Containing 20 mM MgSO4
https://www.enzynomics.com/shop/product_item.php?it_id=206002
