Alw I,10units/μl
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Categories : 3. Protein & Enzyme , Enzynomics (Korea) ,
Brand : Enzynomics (South Korea)
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Recognition & cleavage sequence
Unit definition
One unit is defined as the amount of enzyme required to digest 1 μg of Lambda DNA (dam-) in 1 hour at 37℃ in a total reaction volume of 50 μl.
Reaction condition
- 1X EzBuffer Ⅳ, 37℃
- 1X FastCut Buffer, 37℃
Product component
- Alw I
- 10X EzBuffer IV
- 10X FastCut Buffer
- 6X DNA loading Buffer
- Sterile water
Note
DNA cleavage is blocked by dam methylation. Alw I produces a 5' extension of one nucleotide, which is more difficult to be ligated than blunt-ends. Reaction condition with excess enzyme, excess glycerol (>5%) or extended digestion may result in star activity.
Figure 1. Restriction enzyme activity
1 μg of substrate DNA was digested with increasing amounts of restriction enzyme for 1 hour under appropriate reaction conditions.
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