Afl II,4units/μl
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Categories : 3. Protein & Enzyme , Enzynomics (Korea) ,
Brand : Enzynomics (South Korea)
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Recognition & cleavage sequence
Source: Anabaena flos-aquae
Unit definition
One unit is defined as the amount of enzyme required for complete digestion of 1 μg bacteriophage λ DNA at 37℃ for 1 hr in a 50 μl reaction mixture.
Reaction condition
- 1X EzBuffer IV, 37℃
- 1X FastCut Buffer, 37℃
Thermal inactivation : 65℃
Time : 20 min
Methylation effect
Methylation : Cleavage
dam : Cleavage
dcm : Cleavage
CpG : Cleavage
Product component
- Afl II
- 10X EzBuffer IV
- 10X FastCut Buffer
- 6X DNA loading Buffer
- Sterile water
Note
Afl II is not sensitive to dam, dcm or CpG methylation. Less than 50% of cleaved fragments are ligated in 20-μl reaction mixtures containing 100-500 units of T4 DNA ligase.
Figure 1. Restriction enzyme activity
1 μg of substrate DNA was digested with increasing amounts of restriction enzyme for 1 hour under appropriate reaction conditions.
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