nTaq-multi HOT ,5units/μl,250U

Product description
nTaq-multi HOT is a hot start PCR polymerase which remains inactive at temperatures lower than 75℃. Therefore, a heat activation step is required to activate nTaq-multi HOT. Activation is accomplished at denaturation steps of PCR cycles and, thus, nTaq-multi HOT is active only after initiation PCR cycles. nTaq-multi HOT produces up to 20 different amplified products with a single tube reaction. This product can be used in general multiplex PCR and genotyping experiment related to genetic diagnostics.

Characteristics
- Molecular weight: 94 kDa
- Error rate: 2.4 X 10-5
- Thermal stability: half life of 40 min at 95℃
- A-tail formation at 3’ ends of amplified duplex DNA.
- No activity at temperatures lower than 75℃. Heating up to 95℃ results in activation of enzyme.

Applications
- High specific amplification of DNA fragments shorter than 3kb.
- Amplification of cDNA and genomic DNA.
- Amplification of template DNA with secondary or higher ordered structure that is resistant to PCR amplification
- Well-suited for an automated PCR machines, for which PCR reaction mixtures are prepared at room temperature
- Primer extension
- Multiplex PCR

Supplied with
- 10X nTaq-multi HOT buffer 
- dNTP Mixture (2 mM each)
- GC Melt I
- GC Melt II
- Sterile water

Quality control
- Purity: >99% on SDS-PAGE
- Endonuclease-free
- Exonuclease-free
- RNase free
- Inhibitor-free

Unit definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid insoluble materials in 30 min at 74℃ in a 50-μl reaction mixture (20 mM Tris-HCl/pH 8.8, 50 mM KCl, 2.5 mM MgCl2, 10 mM β-mercaptoethanol, 12.5 μg of calf thymus DNA).

10X nTaq-multi HOT buffer
Containing 15 mM MgCl₂

GC Melt l and ll
- This product is useful for amplification of DNA with GC rich sequences or to avoid amplification of non-specific bands (Use of GC Melt may reduce PCR efficiency).
- In general, use 1X strength in PCR reaction by diluting the 10X solution, but the amount should be adjusted for optimal results.

Cautions
nTaq-multi HOT requires 10 minutes of initial denaturation to ensure effective activation of the enzyme.

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