GENEzol™ TriRNA Bacteria Kit

RNA Extraction/Tri RNAThe GENEzol™ TriRNA Bacteria Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from bacteria samples. Bacterial cell walls are initially lysed using Lysozyme. The sample is then homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

DESCRIPTION
Advantages (Cat. # GZB050, GZB100, GZB200)

• Purify total RNA within 20 minutes without chloroform phase separation or isopropanol RNA precipitation
  
• Sample Volume: bacteria cells up to 1 x 109
  
• Format: phenol, chlorform, guanidine isothiocyanate plus spin column system
  
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
  
• Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, Lysozyme can be
• shipped at room temperature but should be stored at -20ºC for extended periods

APPLICATIONS
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

COMPONENTS

• GENEzol™ Reagent
  
• Pre-Wash Buffer
  
• Lysozyme
  
• Bacteria Lysis Buffer
  
• Wash Buffer
  
• RNase-free Water
  
• RB Columns
  
• 2 ml Collection Tubes

QUALITY CONTROL
The GENEzol™ TriRNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. 5 µl from a 50 µl eluate of purified RNA is analyzed by electrophoresis on a 1% agarose gel.
GENEzol™ TriRNA Bacteria Kit Functional Test Data

Figure 1 .RNA was extracted using the GENEzol™ TriRNA Bacteria Kit. An Escherichia coli (1×109) culture (OD600=2, 1 ml) was harvested by centrifugation at 16,000 x g for 2 minutes, followed by RNA extraction. 5 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

DOCUMENTS

GENEzol™ TriRNA Bacteria Kit

https://geneaid.com/product.php?act=view&id=275