Presto™ Food DNA Extraction Kit

DNA Extraction/Genomic DNA PurificationThe Presto™ Food DNA Extraction Kit is designed for rapid isolation of DNA from a variety of raw and processed food samples. The unique food lysis buffer (containing nonionic detergent CTAB) when combined with Proteinase K is used to digest food tissue and proteins. PCR inhibitors such as polysaccharides and plant metabolites are removed by centrifugation. Chloroform treatment, separates residual inhibitors from the clear supernatant in the organic phase. DNA in the aqueous phase is then bound by the GD Column followed by wash and elution. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 60 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

DESCRIPTION
Specifications (Cat. # FGD100, FGD300)

• Purify genomic DNA from raw or processed food samples within 1 hour!
  
• Sample: 200 mg of food
  
• Binding Capacity: 50 µg of DNA
  
• Format: chloroform phase separation combined with genomic DNA spin columns
  
• Elution Volume: 10-200 µl
  
• Storage: dry at room temperature (15-25°C)

APPLICATIONS
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

• FD1 Buffer
  
• FD2 Buffer
  
• Proteinase K
  
• W1 Buffer
  
• Wash Buffer
  
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  
• GD Columns
  
• 2 ml Collection Tubes

QUALITY CONTROL
The quality of the Presto™ Food DNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 mg food samples. Following the purification process, the purified genomic DNA (A260/A280 ratio 1.8-2.0) is analyzed by electrophoresis.

DOCUMENTS

Presto™ Food DNA Extraction Kit

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