Genomic DNA Maxi Kit (Blood/Cultured Cell)

DNA Extraction/Genomic DNA PurificationThe Genomic DNA Maxi Kit (Blood/Cultured Cell) is optimized for DNA purification (including genomic, mitochondrial and viral DNA) from up to 10 ml of frozen blood or fresh blood and up to 1 x 10⁸ cultured cells using an efficient maxiprep system. This DNA Extraction Kit uses Protease to reduce hemoglobin contamination while chaotropic salt and detergents are used to lyse cells and degrade protein, allowing the DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

DESCRIPTION
Specifications (Cat. # GDM010, GDM025)

• High Yield: up to 200 μg from 10 ml of whole blood
  
• Large Sample Size: up to 10 ml of frozen blood or fresh blood, cultured cells (up to 1 x 108)
  
• Genomic glass fiber maxi spin column optimized for genomic DNA purification
  
• Elution Volume: 500 µl ~ 2 ml
  
• Storage: dry at room temperature (15-25°C)
  
• Operation time: within 1 hour

APPLICATIONS
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

• GB Buffer
  
• W1 Buffer
  
• Wash Buffer
  
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  
• Protease
  
• GD Maxi Columns
  
• Collection Tubes

QUALITY CONTROL
The quality of the Genomic DNA Maxi Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from a 10 ml of whole human blood is extracted using the Genomic DNA Maxi Kit (Blood/Cultured Cell). The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.

DOCUMENTS

Genomic DNA Maxi Kit (Blood/Cultured Cell)

gDNA Maxi Kit (Blood/Cell) Quick Protocol

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