Geneaid™ Maxi Plasmid Kit PLUS (Endotoxin Free)

DNA Extraction/Plasmid DNA PurificationThe Geneaid™ Maxi Plasmid Kit PLUS uses pre-packed anion-exchange resin columns to purify plasmid DNA from 200-1600 ml of cultured bacterial cells. This kit includes an increased volume of reagents for processing 3-6 g bacteria cell pellets. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA is bound to the column and contaminants are efficiently removed. The purified plasmid DNA is eluted then precipitated with isopropanol for desalting. The entire procedure can be completed in 100 minutes without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for transfection, sequencing reactions, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun.

DESCRIPTION
Specifications (Cat. # PME10+, PME25+)

• Includes an increased volume of reagents for processing 3-6 g bacteria cell pellets
  
• High Yield: 1.0 mg of pure transfection grade plasmid DNA from 300 ml cultured bacterial cells
  
• Efficient endotoxin removal (<0.1 EU/µg DNA) verified by LAL
  
• Large Sample Volume: 200-1600 ml of cultured bacterial cells
  
• Format: plasmid maxiprep anion-exchange resin column
  
• Elution Volume: 500 µl - 2 ml
  
• Operation time: within 100 minutes
  
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS
Transfection, Sequencing, Ligation, PCR, In-vitro Transcription Microinjection, Restriction Enzyme Digestion, Gene Gun

COMPONENTS

• PM1, PM2, PM3 Buffer
  
• TrueBlue Lysis Buffer
  
• PER Buffer
  
• PEQ Buffer
  
• PMC Buffer
  
• PEL Buffer
  
• RNase A
  
• Plasmid Maxi Columns

QUALITY CONTROL
The quality of the Geneaid™ Maxi Plasmid Kit PLUS (Endotoxin Free) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 300 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Maxi Plasmid Kit. Following the purification process, a yield of more than 900 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is analyzed by electrophoresis.

DOCUMENTS

Endotoxin Free Maxi Kit PLUS

https://www.geneaid.com/product.php?act=view&id=189