OneScriptPlus cDNA Synthesis Kit,100 x 20 ul reactions
Attribute:
SKU : G236
Categories : 2. Cell & Molecular Biology , DNA / RNA Extraction Kit , ABM Good (Canada) ,
Brand : ABM Good (Canada)
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Description
OneScript® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.
Key Features
- Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
- Robust cDNA synthesis from any RNA template
- High reproducibility and excellent yield
- Employs abm's OneScript® Plus Reverse Transcriptase.
| Product Component | Quantity |
|---|---|
| OneScript® Plus Reverse Transcriptase | 100 rxn (100 µl) |
| 5X RT Buffer | 400 µl |
| Oligo(dT) (10 μM) | 100 µl |
| Random Primers (10 μM) | 100 µl |
| dNTP (10 mM) | 100 µl |
| Nuclease-Free H2O | 2 x 1.0 ml |
SKU G236
Applications
- Synthesizing cDNA from ssRNA
- DNA primer extension
- Sequencing dsDNA
- Constructing cDNA library
- Producing template for use in RT-PCR
- Labelling 3'-end of duplex DNA via end-filling reactions
- Generating probes for hybridization
Storage Condition : Store at -20°C.
Unit quantity : 100 rxn
Material Citation : If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G236
https://www.abmgood.com/onescript-plus-cdna-synthesis-kit.html#G236
