Single Cell Sequencing
Introduction
Dissecting genomic, transcriptomic and epigenomic cellular heterogeneity using single cell Sequencing is essential for understanding biological system. With 10x chromium Gemcode technology system, Macrogen provides a high resolution of individual cell copy number variation, gene expression, cell surface protein expression, chromatin accessibility and immune diversity (TCR, BCR).
Sequencing Platforms
- HiSeq 2500/4000, NovaSeq 6000
Data Analysis*
Single Cell RNA Sequencing
- Standard Data Analysis
- Gene expression and cell clustering analysis
- Simultaneous single cell analysis of gene expression, immune profiling and cell surface protein expression
- Advanced Data Analysis
- Sample aggregation
- Gene expression and cell clustering analysis (Seurat_2.3.4)
- Cell identification
- Differentially Expressed Genes (DEGs) analysis
- Gene Ontology analysis
- Trajectory analysis
Single Cell immune Profiling
- Standard Data Analysis
- Paired full length V(D)J gene sequence identification
- Clonality and diversity of TCR and/or BCR
Single Cell Surface protein Profiling
- Standard Data Analysis
- Surface protein expression and cell clustering analysis
Single Cell AT AC Sequencing
- Standard Data Analysis
- Chromatin accessibility profiles and cell clustering analysis
Single Cell CNV Profiling
- Standard Data Analysis
- Chromatin accessibility profiles and cell clustering analysis
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