SerRed nucleic acid stain (10000×，water-dissolvent), (for Nucleic Acid Electrophoresis)

Product Information

Product Description

SerRed is a highly sensitive, non-mutagenic, ultra-safe and ultra-stable fluorescent nucleic acid gel staining reagent (working concentration), which can replace ethidium bromide (EtBr, EB) with much higher sensitivity than EB without decolorization. SerRed has the same spectral characteristics as EB, and replaces EB without changing the imaging system.

Storage and Shipping Conditions

Ship and store at room temperature; valid for 24 months.

Product Components

Assay Protocol

1. Glue dyeing method (same usage as EB)

a) To prepare the gel, add 5 μL of SerRed nucleic acid dye (10,000 x, water soluble) per 50 mL of agarose (G5056 recommended) gel and mix thoroughly (SerRed has excellent thermal stability and can be added directly to the high-temperature gel solution without waiting for the gel solution to cool. It can also be made by pre-mixing SerRed with an electrophoresis buffer containing agarose powder and heating it).

b) Electrophoresis according to conventional methods.

2. Soak dyeing method

a) Electrophoresis according to conventional methods.

b) Dilute SerRed Nucleic Acid Dye 10,000 x stock solution approximately 3,300 times to make a 3 x staining solution (for example, add 15 μL of SerRed Nucleic Acid Dye 10,000 x stock solution to 50 mL of H2O).

c) Carefully place the gel into a suitable container and soak the gel with a sufficient amount of 3 x staining solution. In order to shorten the soaking time, the dyeing solution can be pre-heated to about 70, then put into the gel and incubate for 10 min to obtain the ideal effect (if not heated, incubate for 30 min in room temperature shaker; if it is acrylamide gel, incubate for 30-60 min and extend with the increase of acrylamide content).The amount of bubble dyeing dye is large, and the dyeing solution can be reused for about 3 times for a single use. The 3×SerRed stain solution can be prepared in large quantities and stored at room temperature until used up.

Note

1. The dye does not need to be refrigerated at low temperature. Please store it at room temperature to avoid precipitation. If precipitation is found, please heat the dyes to 45-50°C for 2 min and shake to dissolve, which does not affect the use.

2. This product can stain single-stranded DNA and RNA, but is less sensitive to single-stranded DNA or RNA than double-stranded DNA.

3. Wear lab coats and disposable gloves during operation.

For Research Use Only!

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