5× SWE Rapid High Resolution Running Buffer, 1 L
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SKU : G2152-1L
หมวดหมู่ : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
แบรนด์ : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
5× SWE Rapid High Resolution Running Buffer | G2152-1L | 1 L |
Product Description
The 5× SWE Rapid High Resolution Running Buffer is optimal to Tris-Glycine electrophoresis buffer for SDS-PAGE gel electrophoresis in Tris-Glycine. Compared with the traditional Tris-glycine running buffer, this product can be used for electrophoresis without adjusting the voltage according to the separation gel and the stacking gel, and it can be used for the whole process of electrophoresis at a constant voltage of 200-250 V, it takes about 30 min to complete the electrophoresis, which saves time and is more effective for the separation of small molecules. It can separate the small molecule protein bands in the prestained protein marker and clearly visible (gel concentration above 8%) to achieve rapid and efficient isolation of proteins. This product needs to be diluted 5 times before use.
Storage and Shipping Conditions
Ship and store at room temperature. Valid for 12 months.
Assay Protocol
1. Mix 100 mL of this product with 400 mL of deionised or distilled water to obtain 500 mL of 1 x working solution.
2. Selection of different protein gel concentrations for different protein molecular weights, refer to the table below:
SDS-PAGE Separation Gel Concentration | Optimum Separation Range (kDa) (SWE Rapid High Resolution Running Buffer) |
6% | 15-300 |
8% | 10-250 |
10% | 5-150 |
12% | 3-100 |
15% | ＜60 |
3. Constant voltage electrophoresis, 200-250 V, refer to the following table for electrophoresis time:
Comparison Table of Electrophoresis Voltage and Electrophoresis Time | |
Voltage(V) | Estimated Electrophoresis Time(min) |
200 | 35 |
220 | 30 |
250 | 25 |
Note
1. It is recommended to use it as soon as possible after opening the lid.
2. This product is suitable for conventional Tris-HCl system SDS-PAGE gels, such as our products G2003, G2037, G2041，G2042，G2043，G2044，G2045，G2060, G2061, G2062, G2063, G2064.
3. This product may have crystal precipitation in the case of low ambient temperature, this is a normal phenomenon, can be heated moderately to help dissolve, to be completely dissolved and cooled to room temperature before use.
4. High voltage electrophoresis is easy to produce high heat. Please select the appropriate voltage for electrophoresis according to the ambient temperature.
5. The running solution can be reused for 2-3 times, more reuse is not recommended.
6. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
