Antibody Stripping Buffer (Plus), 100 mL

Product Information

Product Description

Antibody Stripping Buffer (Plus) is an enhanced reagent that can safely and efficiently remove primary and secondary antibodies from nitrocellulose (NC) and polyvinylidene fluoride (PVDF) membranes to facilitate the redetection of chemiluminescence protein immunoblotting.

Save time and effort, eliminate errors: No need to re-gel, sample and electrophoresis; save precious samples; eliminate re-sampling errors.

Quick and efficient: Elution time 5 - 15 min at room temperature; stronger elution ability than ordinary type.

Odorless: Thiol-free, no offensive odors.

Ready and easy to use: No dilution necessary, ready to use with open cap.

Storage and Shipping Conditions

Ship with wet ice, store at 2-8, valid for 12 months.

Components

Assay Protocol

1. After the first chemiluminescence detection, TBST rinse for 5min.

2. Pour off the TBST, add sufficient Antibody Stripping Buffer (Plus) to cover the NC or PVDF membranes and incubate for 5 to 15 minutes at room temperature with gentle shaking.

3. Remove the blot from the Antibody Stripping Buffer (Plus) and wash 3 times for 5 minutes each in TBST.

4. Follow-up work such as blocking and antibody incubation is performed according to the purpose of the experiment.

Note

1.  This product is corrosive, take caution when using it.

2. Reuse is not recommended.

3. The membrane will become transparent when in use, and will return to normal after cleaning.

4.  For your safety and health, please wear safety glasses, gloves, or protective clothing.

Table 1: Reference for antibody stripping buffer selection

For Research Use Only!

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