Products
Pathological Reagents | Consumables
Morphology Staining Solution
Special Stains
Nerve Tissue Stain
Luxol Fast Blue (Myelin Sheath) Stain, 100 mL×3 (for Nervous tissue staining)
คุณสมบัติสินค้า:
SKU : G1030-100ML
หมวดหมู่ : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
แบรนด์ : Servicebio
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Product Information
Product Name | Cat.No. | Spec. |
Luxol Fast Blue Myelin Sheath Stain Reagent | G1030-100ML | 3×100 mL |
Description
Myelin sheath (myelin sheath) is a tubular sheath structure wrapped around the outside of a nerve axon, which is segmental in nature, has an insulating effect and improves the conduction velocity of nerve impulses, and acts as a protector of axons in the central and peripheral nervous system. Myelin sheath is made up of sphingomyelin, which consists mainly of lipids and proteins. Myelin staining has certain pathological diagnostic significance. If myelin is diseased, the number of myelin is significantly reduced or disappeared during staining, or the shape of myelin is irregular.
The main components of this product are as follows: myelin dye solution A is 0.1% solid blue (Luxol fast blue), dye solution B is lithium carbonate differentiation solution, and dye solution C is 70% ethanol. After staining with this product, the nerve myelin appears blue with a light blue background or almost colorless.
Storage and Handling Conditions
Store and transport at room temperature, valid for 18 months.
Component
Component number | Component | G1030-100ML |
G1030-1 | Luxol fast blue solution A | 100 mL |
G1030-2 | Luxol fast blue solution B | 100 mL |
G1030-3 | Luxol fast blue solution C | 100 mL |
Manual | 1 pc | |
Assay Protocol
1. The paraffin sections are dewaxed to water.
2. Preheat myelin dye solution A in advance at 65 oven for 30 min. Tissue sections were stained in preheated myelin dye solution A for 4 h at 65 ° C (capped to prevent evaporation of the liquid).
3. Remove the slides after cooling at room temperature and wash with tap water until the slides are colorless.
4. The sections were quickly immersed in myelin staining solution B for rapid differentiation for 5 s, and then the sections were immediately placed in myelin staining solution C for rapid differentiation for 10 s. Even if the sections were alternatively immersed in myelin staining solution B and myelin staining solution C for differentiation, the sections were examined timely until the myelin sheaths showed blue background and nearly colorless, and then the differentiation was terminated by washing.
5. The slices were dehydrated with absolute ethanol for 3 times, 5 min each time, transparent with xylene for 5 min, and then sealed with neutral gum.
Note:
1. Preheating of Luxol fast blue solution A is very important, and the temperature of solution will have a certain impact on the strength of tissue coloring.
2. After the sections are stained and washed with solution A, they should be differentiated as soon as possible to avoid dry tissue.
3. Each set of staining solution can be used for staining (dip staining) approximately 60 sections. Replace the stain with a new one when the tissue or cell coloring is significantly lighter or abnormal.
4. Wear a lab coat and disposable gloves during operation.
For Research Use Only!
