Goldner trichome Stain, 20 mL×6 (for Bone staining)

Product Information

Description

Goldner trichromatic staining solution, similar to Masson trichromatic staining, usually refers to staining of nuclei and selectively displaying collagen and muscle fibers. The dyeing principle is related to the size of the anionic dye molecules and the permeability of the tissues. The dyes with small molecular weight can easily penetrate the tissues with dense structure and low permeability, while the dyes with large molecular weight can only enter the tissues with loose structure and high permeability. This product is mainly used for staining bone tissue sections, which can distinguish mineralized bone from non-mineralized bone (osteoid). The collagen fibers in mineralized bone undergo conformational changes during mineralization and deposition of inorganic salts. Thus, after staining, mineralized bone is stained green, non-mineralized bone (osteoid) is stained orange-red, and the nucleus is blue-purple.

Storage and Handling Conditions

Store and transport at room temperature from light; valid for 12 months.

Component

Assay Protocol

1. Paraffin sections shall be dewaxed and rehydrated according to conventional steps.

2. Before use, mix equal volumes of Goldner staining solution A and Goldner staining solution B according to the dosage. This mixture is ready for use and can be prepared as needed. The sections were stained in the mixture for 20 min, washed with tap water, and then rapidly differentiated with 1% hydrochloric acid alcohol for 2 s. The sections were washed with tap water and distilled water.

3. The sections were stained with Goldner staining solution C for 5-10 min, and then rinsed twice with 0.2% glacial acetic acid for 3-5 s each time.

4. The sections were immersed in Goldner staining solution D for 3 min and observed under a microscope. At this time, the red color of the collagen should fade.

5. The sections were immersed in Goldner dye solution C again for 3-5 min, and rinsed quickly with 0.2% glacial acetic acid twice, 3-5 s each time.

6. Sections were immersed in Goldner solution E staining for 3-5 min, and then rapidly differentiated with 0.2% glacial acetic acid for 3 times, 2 s each time. Then it was rapidly dehydrated with absolute ethanol for 3 s and 5 s, respectively.

7. Transparent sealing: The slices were dehydrated with absolute ethanol for 5 min, transparent with xylene for 5 min, and sealed with neutral gum.

Note

1. The staining degree of Goldner staining solution C and Goldner staining solution D should be controlled according to the staining depth. The collagen part should not be too red, which will affect the coloring of Goldner staining solution D.

2. After Goldner staining solution E staining, attention should be paid to controlling the degree of differentiation to avoid insufficient or excessive differentiation.

3. Wear a lab coat and disposable gloves during operation.

For Research Use Only!

https://www.servicebio.com/goodsdetail?id=1794