20×SSC buffer solution, 100 mL (for Nucleic Acid Electrophoresis)

Product Information

Product Description/Introduction

SSC (Saline sodium citrate) buffer is a classic blotting and hybridization treatment solution in molecular biology. It is used for denaturation and washing in various hybridization experiments. The main components are sodium chloride and sodium citrate. The sodium citrate acts as a buffer and the salt ion binds to negatively charged nucleic acids making them electrically neutral and facilitating the binding of the probe to the target sequence. SSC solution can also be used for the preparation of SDS-PAGE electrophoresis separation gel for nucleic acid hybridization. Commonly used in concentrations of 2× and 0.5×. The 2×SSC is usually used to wash the membrane with high salt to wash away some nonspecific bound probes. The 0.5×SSC is often used to wash the membrane with low salt to increase the tightness of nucleic acid chain and the repulsion between RNA / DNA. The product is 20× concentrated solution, pH 7.0, prepared with pyrogen-free ultrapure water and filtered by microporous membrane for sterilization. The main ingredients are 3.0 M sodium chloride and 0.3 M sodium citrate.

Storage and Shipping Conditions

Ship and store at room temperature, valid for 24 months.

Assay Protocol/Procedures

According to the application of requirements, it is recommended to dilute with sterile water.

Note

1. This product is a concentrated solution and needs to be diluted before use. The diluted buffer solution is recommended to be used up on the same day and can be temporarily stored at 4.

2. Pay attention to avoid microbial contamination during using to prevent any influence on the experimental results.

3. For your safety or health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!

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