Recombinant Human CD19 Protein (His-tag)

Gene Name Synonym

CD19, B4, CVID3, MGC12802

Species

Human

Accession

P15391

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Pro20-Lys291 is expressed with a 10×His tag at the C terminus

Molecular Weight

22 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It is a biomarker for lymphoma diagnosis, B lymphocyte development and can be used as a target for leukemia immunotherapies. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human CD19 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 40-65 kDa.

Immobilized Human CD19, His Tag at 5 μg/mL (100 μL/well) can bind Anti-CD19 with a linear range of 0.39-3.12 ng/mL.