Mycoplasma Elimination Reagent (100×), 10×1 mL

Product Information

Product Description/Introduction

This product for cellular mycoplasma removal is a combination of quinolones and tetracyclines that interfere with mycoplasma DNA replication and protein synthesis, respectively, resulting in effective removal of cellular mycoplasmas. This product has low toxicity to eukaryotic cells, and can completely remove the infected mycoplasma in about 10 days. After replacing the normal medium, the cells are in good condition, and there is no re-positive phenomenon in continuous multi-generation culture.

Storage and Shipping Conditions

Ship with wet ice; store at -20, avoid freeze -thaw cycles, valid for 12 months.

Product Contents

Assay Protocol / Procedures

1. Add 1 mL of Mycoplasma Elimination Reagent (100×) to 100 mL of cell culture medium (containing serum, without double antibiotic) and mix well to obtain the medium containing Mycoplasma Elimination Reagent.

2. Replacement of Mycoplasma Elimination Reagent-containing medium for routine cell culture during passaging or fluid exchange of mycoplasma-infected cells. 

3. After one week of culture, planting some cells into 6-well plates (or other standard culture vessels) and cultured with normal culture medium. The remaining cells are continued to be cultured with medium containing Mycoplasma Elimination Reagent.

4. Cells in 6-well plates are cultured with normal culture medium for 2-3 days, and mycoplasma detection (recommended G1900, G1901, G1902) is performed when the cell confluence reaches more than 90%.

5. According to the test result, perform the following operations:

A) Positive result: continue to repeat steps 3-4 until the test result is negative.

B) Negative results: the cell group containing Mycoplasma Elimination Reagent medium is replaced with normal complete medium, and Mycoplasma detection is performed after a period of culture; A negative retest indicates that mycoplasma elimination is complete.

Note

1. Avoid repeated freezing and thawing. If you need to use multiple times, please pay attention to appropriate packaging. In addition, if there is crystallization or precipitation after thawing, vortex or reverse mixing can promote crystallization or precipitation dissolution, without affecting the use effect.

2. This product has been tested in a variety of mycoplasma infected cell lines, but different cell lines have different sensitivity to reagents. If the cytotoxicity is too great or the removal effect is not good, the solubility of the product can be adjusted appropriately, and other scavenger can be selected (recommend G4007, G4008).

3. It is recommended to add mycoplasma prevention reagent (recommend G4009) in cell cultures to prevent mycoplasma contamination.

4. For cells that have been identified as mycoplasma contamination, a separate ultra-clean bench should be used to avoid cross-contamination.

5. For your safty and health, please wear safety glasses, gloves, or protective clothing.

Attached Table: Features of Mycoplasma Removal Series

For Research Use Only!

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