SKU : MB-M1191
หมวดหมู่ : 12. Lab Equipment ,  MB Cell , 
แบรนด์ : MB cell
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M9 Minimal Broth [MB-M1191]
Medium used for cultivation and enumeration of E.coli for molecular biology purpose.
PROCEDURE
Suspend 10.5 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45-50°C in water bath. Aseptically add 2 vials of M9 Minimal Broth supplement(MB-M0732). Mix well. Dispense in tubes.
STORE
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8°C.