Product descriptionnTaq is a recombinant protein from the Thermus aquaticus DNA polymerase I gene expressed in E. coli. The Taq DNA polymerase is thermostable and has 5'→3' exonuclease activity but lacks 3'→5' exonuclease activity. nTaq is a variant of Taq DNA polymerase. nTaq is modified in the N-terminal domain to deplete 5'→3' exonuclease activity, which sometimes causes erroneous amplification. nTaq is additionally modified in the polymerase domain to improve amplification of high-GC and secondary-structured regions of DNA. nTaq activity is optimal at 72℃
Characteristics
- Molecular weight: 94 kDa
- Error rate: 2.4 × 10-5
- Thermal stability: half-life of 40 min at 95℃
- A-tail formation at 3’ ends of amplified duplex DNA
Applications
- Amplification of DNA fragments shorter than 3 kb (suitable for general PCR applications)
- Genomic DNA or cDNA template
- Primer extension
- Colony PCR
- Radioactive labeling of DNA
- Nucleotide sequencing
Quality control
- Purity: >99% on SDS-PAGE
- Endonuclease-free
- Exonuclease-free
- RNase-free
- Inhibitor-free
Supplied with
- 10X nTaq buffer (Mg2+ plus)
- dNTP Mixture (2 mM each)
- Sterile water
Unit definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid insoluble materials in 30 min at 74℃ in a 50-μl reaction mixture (20 mM Tris-HCl/pH 8.8, 50 mM KCl, 2.5 mM MgCl2, 10 mM β-mercaptoethanol, 12.5 μg of calf thymus DNA).
10X nTaq Buffer
Mg2+ plus buffer: containing 15 mM MgCl2
Figure 1. Amplification of various human DNA fragments with nTaq DNA polymerase.
20 different DNA fragments were amplified with nTaq DNA polymerase (Cat. P025 or P050). Target DNAs with varying lengths were amplified by the use of following PCR cycles; 95℃ 2 min, (95℃ 30 sec, 55℃ 30 sec, 72℃ 1 min) x 30, 72℃ 5 min.
Lane 1~20, 120bp~ 1Kb; M, 1 kb (+) Ladder Marker (Cat. DM003)
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