nTaq-Tenuto (Mg2+ plus) ,5units/μl,250U

Product description

nTaq-Tenuto is nTaq (Cat. P025, P050) supplemented with 3'->5’ proofreading activity and a PCR enhancing factor for improved efficiency and fidelity. nTaq-Tenuto can be used to amplify DNA longer than 10 kb, which is difficult with common Taq polymerases alone. Thus, this product is improved in both fidelity (> 2 fold) of PCR products and amplification efficiency of longer PCR products.

Characteristics

- Molecular weight: 94 kDa

- Error rate: 3.0 X 10^-6

- Thermal stability: Half life of 40 min at 95℃

- A-tail formation at 3’ ends of amplified DNA products.

Applications

- Amplification of long DNA fragments (<10 kb)

- Amplification of high-complexity template DNA

- Primer extension

- Colony PCR

- Labeling of DNA fragments with radioactive-isotopes

- Nucleotide sequencing

Supplied with

○ nTaq-Tenuto (Mg²⁺ plus buffer)

- 10X nTaq-Tenuto buffer (Mg²⁺ plus)

- dNTP Mixture (2 mM each)

- GC Melt I

- GC Melt II

- Sterile water

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase free

- Inhibitor-free

Unit definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid insoluble materials in 30 min at 74℃ in a 50-μl reaction mixture (20 mM Tris-HCl/pH 8.8, 50 mM KCl, 2.5 mM MgCl₂, 10 mM β-mercaptoethanol, 12.5 μg of calf thymus DNA).

10X nTaq-Tenuto buffer

Mg²⁺ plus buffer: Containing 20 mM MgCl₂

GC Melt l and ll

- This product is useful for amplification of DNA with GCrich sequences or to avoid amplification of non-specific bands (Use of GC Melt may reduce PCR efficiency).

- In general, use 1X strength in PCR reaction by diluting the 10X solution, but the amount should be adjusted for optimal results.