Magnetic Beads DNA Extraction Kit (Bacteria)

Magnetic Beads DNA/RNA Extraction/Magnetic Beads gDNA kit BacteriaThe Magnetic Beads Genomic DNA Extraction Kit Bacteria was designed specifically for efficient genomic DNA purification from Gram (-) negative and Gram (+) positve bacteria. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and a variety of other downstream applications.

DESCRIPTION
Advantages (Cat. # ME048, ME096)

• High Yield: >26 µg of Genomic DNA
  
• High Quality DNA: A260/A280 = 1.8-2.0
  
• Magnetic Bead Concentration: 50 mg/ml
  
• Magnetic Bead Size: ~ 5 µm
  
• Easily adapted to automated magnetic bead separation instruments and workstations
  
• Sample: 2 x 109 of bacteria cells (Gram + and Gram -)
  
• Manual or automated DNA isolation
  
• Operation time: within 30 minutes (manual) 
  
• Storage: dry at room temperature (15-25ºC), Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods

APPLICATIONS
qPCR, PCR, Real-time PCR, Automated Fluorescent DNA Sequencing, Southern Blotting, Next Generation Sequencing (NGS)

COMPONENTS

• ME1 Buffer
  
• ME2 Buffer
  
• ME3 Buffer
  
• ME4 Buffer
  
• Lysozyme
  
• Proteinase K
  
• MW1 Buffer
  
• MW2 Buffer
  
• ME Magnetic Beads
  
• Elution Buffer

QUALITY CONTROL
The quality of the The Magnetic Beads Genomic DNA Extraction Kit Bacteria is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating genomic DNA from 1 x 10⁹ cells of Escherichia coli and from  1 x 10⁹ cells of Bacillus subtilis. 5 μl of extracted DNA product from a 100 μl eluate is analyzed on a 0.8% agarose gel.

DOCUMENTS

Magnetic Beads gDNA Kit (Bacteria)

https://geneaid.com/Magnetic-Beads-gDNA-kit-Bacteria/ME