Presto™ cfDNA/RNA Extraction Kit

DNA/RNA/Protein Extraction/Presto™ cfDNA/RNA Extraction KitThis miniprep cfDNA/cfRNA kit was designed for circulating cell-free DNA/RNA purification from up to 5 ml of serum or plasma. The cfDNA/cfRNA extraction kit includes uniquely designed Column Extension Tubes which allow for increased sample volume and yield. Biological liquid samples are lysed using Proteinase K and a buffer containing chaotropic salt. The lysate is mixed with a binding buffer to facilitate cfDNA/RNA binding to the column membrane. The column is then washed and cfDNA/RNA is eluted with RNase-free Water. The entire procedure can be completed within 60 minutes and the purified cfDNA/RNA is ready for use in a variety of downstream applications such as qPCR, Next-Generation sequencing and DNA methylation analysis.

DESCRIPTION
Advantages (Cat. # CF025, CF050, CF100)

• Samples: 1-5 ml of serum or plasma
  Yield: 1-100 ng
  
• Includes column extension tubes for increased sample volume and yield
  
• Elution Volume: 30-50 μl
  
• Storage: dry at room temperature (15-25°C)

APPLICATIONS
qPCR, Next-Generation sequencing and DNA methylation analysis

COMPONENTS

• CF1 Buffer
  
• CF2 Buffer
  
• Proteinase K
  
• W1 Buffer
  
• Wash Buffer
  
• RNase-free Water
  
• Carrier RNA
  
• Column Extension Tubes
  
• CF Column
  
• 2 ml Collection Tubes

QUALITY CONTROL
The quality of the Presto™ cfDNA/RNA Extraction Kit is tested on a lot-to-lot basis by isolating cfDNA/RNA from 1 ml of plasma. Following the purification process, the purified cfDNA/RNA integrity is assessed by qPCR.

Presto™ cfDNA/cfRNA Kit Interactive Protocol

DOCUMENTS

Presto™ cfDNA/RNA Extraction Kit

https://geneaid.com/RNA-Extraction-Kit/CF