Presto™ Mini RNA Bacteria Kit w/DNase Set

RNA Extraction/Total RNAThe Presto™ Mini RNA Bacteria Kit was designed for total RNA purification from Gram (-) negative bacteria and Gram (+) positive bacteria using an efficient RNA miniprep system. This RNA Kit includes Bacteria Lysis Buffer and Lysozyme to reduce sample preparation time and minimize hands on time. The Presto™ Mini RNA Bacteria Kit uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column and once any contaminants have been removed, using the Wash Buffer, the purified total RNA is eluted by RNase-free Water. High quality total RNA can be purified within 20 minutes and is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

DESCRIPTION
Specifications (Cat. # RBBD050, RBBD100, RBBD300)

• Purify total RNA within 20 minutes!
  
• High Yield: up to 60 μg of pure RNA (1 x 109 Escherichia coli: 40-45 µg, 1 x 109 Bacillus subtilis: 50-55 µg)
  
• Wide Variety of Bacteria Sample Strains: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 109)
  
• Convenient: Includes Bacteria Lysis Buffer and Lysozyme
  
• Spin Columns: glass fiber membrane optimized for total RNA extraction
  
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  
• Storage: dry at room temperature (15-25°C), Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods

APPLICATIONS
RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis

COMPONENTS

• Bacteria Lysis Buffer
  
• Lysozyme
  
• RB Buffer
  
• DNase I
  
• DNase I Reaction Buffer
  
• W1 Buffer
  
• Wash Buffer
  
• RNase-free Water
  
• RB Columns
  
• 2 ml Collection Tubes

QUALITY CONTROL
The quality of the Presto™ Mini RNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified RNA from Escherichia coli cells (up to 1 x 109), (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Presto™ Mini RNA Bacteria Kit Functional Test Data

Figure 1 .RNA was extracted using the Presto™ Mini RNA Bacteria Kit. An Escherichia coli (1×109) culture (OD600=1.3, 1 ml) was harvested by centrifugation at 16,000 x g for 1 minute. 10 µl from a 50 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel.

Figure 2 .Escherichia coli RNA was purified using Presto™ Mini RNA Bacteria Kit. The integrity and quality of RNA were determined by Qubit with RNA integrity and quality (IQ) assay kit. The IQ 9.1 score showed 91% large and structured RNA (including mRNA, tRNA and rRNA) was in the purified RNA.

DOCUMENTS

Presto™ Mini RNA Bacteria Kit

PrestoTM Mini RNA Bacteria Kit Quick Protocol

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