Total RNA Mini Kit (Tissue) w/DNase Set

RNA Extraction/Total RNAThe Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. The Total RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes and DNase I for DNA sensitive downstream applications.

DESCRIPTION
Specifications (Cat. # RTD050, RTD100, RTD300)

• Purify total RNA within 15 minutes!
  
• Sample: up to 25 mg of tissue (tailsnips, liver, kidney, brain, spleen, earpunches etc.)
  
• High Yield: 5-30 μg of pure RNA
  
• RNase and DNase-free Micropestles included for simple tissue homogenization within the tube to reduce the risk of sample contamination
  
• Spin Columns: glass fiber membrane optimized for total RNA extraction
  
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  
• Storage: dry at room temperature (15-25°C), DNase I is shipped at room temperature and should be stored at -20°C

APPLICATIONS
RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protection Assay

COMPONENTS

• RB Buffer
  
• DNase I
  
• DNase I Reaction Buffer
  
• W1 Buffer
  
• Wash Buffer
  
• RNase-free Water
  
• RB Columns
  
• Filter Columns
  
• 2 ml Collection Tubes
  
• Micropestles

QUALITY CONTROL
The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total RNA from a 25 mg fresh tissue sample is extracted using the Total RNA Mini Kit (Tissue). The purified RNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Total RNA Mini Kit (Tissue) Functional Test Data

Figure 1 .Total RNA was extracted from 10 mg of various mouse tissue using the Total RNA Mini Kit (Tissue). 5 μl aliquots of each sample was analyzed by electrophoresis on a 1% agarose gel.

1. Brain, 2. Lung, 3. Liver, 4. Spleen, 5. Kidney

DOCUMENTS

Total RNA Mini Kit (Tissue)

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