GenepHlow™ Gel Extraction Kit

DNA Extraction/Gel ExtractionThe GenepHlow™ Gel Extraction Kit was designed to recover or concentrate DNA fragments from agarose gel. This gel extraction kit includes QG Buffer premixed with a pH indicator to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.

DESCRIPTION
Specifications (Cat. # DFG100, DFG300)

• High Efficiency: up to 90% recovery from agarose gels
  
• Only 20 minutes gel extraction
  
• Convenient: QG binding buffer is premixed with a pH indicator to allow for easy determination of optimal pH
  
• Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high following gel dissociation
  
• Sample Size: up to 300 mg of agarose gel slice
  
• Broad Fragment Size Range: 70 bp–20 kb
  
• Spin Columns: glass fiber membrane optimized for gel extraction
  
• Elution Volume: 20-50 µl
  
• Storage: dry at room temperature (15-25°C)

APPLICATIONS
Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection

COMPONENTS

• QG Buffer (premixed with a pH indicator)
  
• 3M Sodium Acetate (pH5.0)
  
• W1 Buffer
  
• Wash Bufffer
  
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  
• DFH Columns
  
• 2 ml Collection Tubes

QUALITY CONTROL
The quality of the GenepHlow™ Gel Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from agarose gel and analyzed by electrophoresis.

DOCUMENTS

GenepHlow™ Gel Extraction Kit

GenepHlow™ Gel Extraction Kit Quick Protocol

GenepHlowTM Gel Extraction Kit 琼脂糖凝胶回收试剂盒

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