SYNONYMS
Thioredoxin mitochondrial, Thioredoxin-2, TXN2, MTRX, TRX2, MT-TRX, TRX-2, TXN-2.
TYPE
Mouse Anti Human Monoclonal.
INTRODUCTION
Thioredoxin-2 is a low molecular weight redox protein. TRX2 contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The TXN2 is involved in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. Upon stimulation of Fas, TXN2 mediates denitrosylation of mitochondria-associated caspase-3, a process required for caspase-3 activation, and promoted apoptosis.
TRX2 is important at low oxidative stress conditions.
MTRX is involved in the regulation of the mitochondrial membrane potential and cell death. Mitochondrial thioredoxin plays an important roles in protection against oxidant-induced apoptosis. Thioredoxin1 and thioredoxin2 have opposed regulatory functions on hypoxia-inducible factor-1alpha.
CLONE
PAT1B1AT.
PHYSICAL APPEARANCE
Sterile filtered colorless solution.
IMMUNOGEN
Anti-human TXN2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TXN2 protein 60-166 amino acids purified from E. coli.
IG SUBCLASS
Mouse IgG2b heavy chain and k light chain.
PURIFICATION METHOD
TXN2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
FORMULATION
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
STORAGE PROCEDURES
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.
Prevent freeze thaw cycles.
STABILITY / SHELF LIFE
12 months at -20°C. 1 month at 4°C.
APPLICATIONS
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
SAFETY DATA SHEET
SDS
USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
