IL7, Human

PRODUCT DESCRIPTION
GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Recombinant Human Interleukin-7
IL7
Pre-B-cell growth factor (PBGF)

Interleukin-7 (IL7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL7 receptor, a heterodimer consisting of IL7 receptor alpha and IL2 receptor gamma chains. IL7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells. IL7 can also stimulate the proliferation of B-cells, T cells and NK cells (natural killer cells). Murine IL7 has approximately 61% amino acid sequence identity with human IL7, and both proteins exhibit cross-species activity. IL7 has been examined as an immunotherapy agent in many human clinical trials for various malignancies, along with its role in HIV-positive individuals.

Formulation: Sterile filtered white lyophilized powder. Purified and tested for use in cell culture.

Sequence: DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EH

MW: ~17.4 kDa (152 amino acids)

Purity: >97% by SDS-PAGE and HPLC analyses.

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than0.5 ng/ml, corresponding to a specific activity of >2.0 × 106 IU/mg.

Accession Number: P13232

HGNC ID: 6023

Source: Escherichia coli

Storage/Handling: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.

Reconstitution: The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a siliconized tube using PBS that contains a 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions containing BSA or serum.

Additional resources for this product may be found at: OMIM

NOT FOR HUMAN USE!