Oligonucleotide Synthesis

บริการสังเคราะห์ไพร์เมอร์ (Primer Synthesis)


Macrogen provides the best oligo synthesis service with the latest equipment and an automated system.

High-quality oligos are produced via a purification process by removing impurities and
oligos that are nonspecifically bound. The quality of all oligos is controlled by MALDI-TOF mass spectrometry.

Features

· Scales from 0.05 umoleto 1.0 umole
· Quick delivery by express courier (using FedEx / DHL International shipping)
· Accurate concentration provided by the Liquid Handling System
· Orders, progress of synthesis, delivery, and results of synthesis can be confirmed
    by Laboratory Information Management System (LIMS)

Work Flow

Order Confirmation  Oligo Synthesis  Quality Check  Delivery

Refined Quality

MOPC
(Macrogen Oligonucleotide Purification Cartridge)
HPLCPAGE

- Available for both standard oligos and some modified oligos
- Purity of ≥ 85 % (LC Analysis)
- Available for free as a default for all oligos

- Available for all standard oligos and modified oligos
- Recommended for oligos of 50 mer or less
- Purity of ≥ 90 % (LC Analysis)		- Available for all standard oligos and modified oligos
- Recommended for oligos of 50 mer or less
- Purity of ≥ 95 % (LC Analysis)


100% Quality Control(OD/MALDI-TOF)

· Standard Oligo : Can be synthesized for 3 ~ 130 mer(However, it is not provided with MALDI Q/C after not more than 5mer or 90mer)
· Modified Oligo : Up to 60 mer is recommended(Inquire for oligos of 60 mer or more)

Guaranteed Yield(OD)Synthesis Scale(25mer 기준)
0.05 umole0.2 umole1 umole
MOPC(Standard Oligo)6820
MOPC(Modified Oligo)-510
PAGE248
HPLC248

Release and delivery of synthesized oligos occur after OD measurement
and MALDI-TOF MS analysis to ensure quality. The result report is shipped with the ordered oligos.

Use MALDI-TOF mass spectrometry equipment to accurately analyze Oligo and identify Oligo,
which includes N-1, N-2 mers, etc.

ราคาการสั่งสังเคราะห์ไพร์เมอร์

Synthesis Scale (ฺBase)Price/Base* (THB) 
0.025 µmole0.05 µmole0.2 µmole1 µmole
1-50 Base10 1850100
51-299 Base9174794
>300 Base8154388
Purification (Oligo)Price/Oligo* (THB) 
DesaltFree---
MOPC-FreeFreeFree
PAGE-125012501900
HPLC-125012501900


Modified Oligos

Various types of modification oligo synthesis services are provided using high-quality raw materials and optimized processes.
Various scale selections allow HPLC and PAGE purification, and some MOPC refining.

Modification Type

Fluorescent Dyes- FAM, HEX, TET, JOE, TAMRA, CY3, CY5, CY3.5, CY5.5
- CAL Fluor560, CAL Flour610
- ATTO565 NHS-ester, ROX NHS-ester, TexasRed NHS-ester
- Yakima Yellow
Non Florescent Modifications- Phosphorylation, Biotin, NH2C6, NH2C12, C3 Spacer, dSpacer
- Thiol C6 S-S
- Biotin-TEG
Dark Quenchers- Black Hole Quencher(BHQ1, BHQ2, BHQ3)
- Blackberry Quencher(BBQ650)
- Dabcyl and Eclipse Quencher
Internal Modification- Deoxylnosine(dl)
- DeoxyUridine(dU)
- Phosphorothioate(PS bond)
- C3 Spacer, dSpacer, Thiol C6 S-S
- CY3. CY5

3' Modification

ModificationPrice/Primer* (THB)
0.2 µmole1.0 µmole
3’C3 Spacer2,7004,000
3’Phosphorylation1,6002,700
3'Biotin2,1004,800
3'Biotin-TEG3,7005,600
3'BHQ13,4007,400
3'BHQ23,4007,400
3'BHQ35,8009,200
3'Dabcyl5,0007,900
3'BBQ6505,3008,200
3'Eclipse9,00011,100
3'FAM3,5006,900
3'CY35,8008,400
3'CY55,8008,400

 5' Modification

ModificationPrice/Primer* (THB)
0.2 µmole1.0 µmole
5'Phosphorylation1,6003,200
5'NH2 C61,4003,200
5'NH2 C123,2007,400
5'Biotin3,70010,500
5'Biotin-TEG4,2005,800
5'Thiol C6 S-S5,3008,700
5’C3 Spacer2,7004,000
5’dSpacer4,5007,400 
5'FAM2,0004,500
5'HEX2,9006,600
5'TET2,9006,600
5'TAMRA2,9006,600
5'JOE3,5007,400
5'CY36,3007,400
5'CY3.59,20014,200
5'CY56,3007,900
5'CY5.59,20014,200
5'CAL Fluor56010,80015,500
5'CAL Fluor61010,80015,500
5'ATTO565 NHS-ester10,80015,500
5'ROX NHS-ester9,20013,200
5'TexasRed NHS-ester11,60016,800
5'YakimaYellow7,70010,300


Internal Modification

ModificationPrice/Primer* (THB)
0.05 µmole0.2 µmole1.0 µmole
DeoxyInosine
(dI)		500600600
DeoxyInosine
(dU)		400600800
Phosphorothioate200250300

 Duplex Oligo (double stranded oligos)

ModificationPrice/Primer* (THB)
0.05 µmole0.2 µmole1.0 µmole
Double stranded oligos1,1001,6001,900

MGB Probe

ModificationPrice/Primer* (THB)
0.2 µmole1.0 µmole
5'FAM-3'MGBEclipse21,00031,500
5'HEX-3'MGBEclipse26,30039,200
5'TET-3'MGBEclipse26,30039,200

 Dual-Labeled DNA Probes

ModificationPrice/Primer* (THB)
0.2 µmole1.0 µmole
5'ATTO565-3'BHQ118,90027,300
5'ATTO565-3'BHQ218,90027,300
5'ATTO565-3'BBQ65021,60030,500
5'ROX-3'TAMRA14,50021,800
5'ROX-3'BHQ215,30022,600
5'ROX-3'BBQ65017,40025,000
5'TexasRed-3'BHQ118,20026,000
5'TexasRed-3'BHQ218,20026,000
5'TexasRed-3'BBQ65017,90026,000
5'YakimaYellow-3'BHQ114,70023,700
5'YakimaYellow-3'BHQ214,70023,700
5'YakimaYellow-3'Dabcyl14,70023,700
5'FAM-3'TAMRA6,30013,200
5'TET-3'TAMRA7,40015,800
5'HEX-3'TAMRA7,40015,800
5'CY3-3'BHQ111,60020,500
5'CY3-3'BHQ211,60020,500
5'CY3-3'BHQ311,60020,500
5'CY3.5-3'BHQ218,40026,000

Dual-Labeled DNA Probes

ModificationPrice/Primer* (THB)
0.2 µmole1.0 µmole
5'CY5-3'BHQ211,30016,300
5'CY5-3'BHQ311,60020,500
5'CY5.5-3'BHQ216,80025,000
5'CY5.5-3'BHQ316,80025,000
5'CY5.5-3'BBQ65017,60025,800
5'FAM-3'BHQ16,30013,700
5'HEX-3'BHQ17,40014,700
5'HEX-3'BHQ28,40014,200
5'TAMRA-3'BHQ110,50020,000
5'TAMRA-3'BHQ210,50020,000
5'JOE-3'TAMRA12,60021,000
5'JOE-3'BHQ112,60021,000
5'JOE-3'BHQ212,60021,000
5'TET-3'BHQ112,10023,100
5'TET-3'BHQ212,10023,100
5'FAM-3'Dabcyl9,50014,500
5'HEX-3'Dabcyl11,10018,900
5'TET-3'Dabcyl12,10024,200
5'TAMRA-3'Dabcyl12,10024,200
5'JOE-3'Dabcyl12,60024,700
5'CAL Fluor560-3'BHQ114,00025,500
5'CAL Fluor610-3'BHQ214,00025,500
5'CAL Fluor610-3'Dabcyl14,00025,500


 

* ราคายังไม่รวม vat 7%

หมายเหตุ รอบการสั่งสังเคราะห์ทุกวันพฤหัสของสัปดาห์ ระยะเวลาได้รับสินค้าไม่เกิน 7-14 วัน

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