Transcriptome Sequencing
Introduction
Transcriptome sequencing using Next Generation Sequencing is a fast and reliable method for identifying genomic information. Macrogen provides whole mRNA transcript expression analysis, enabling novel gene discovery, identification of novel SNP and lnDel, novel splice variant and chromosomal rearrangement discovery, and identifications of fusion genes.
Sequencing Platforms
- HiSeq 2500/4000, NovaSeq 6000
Data Analysis*
De Novo Sequencing
- Standard Data Analysis
- De novo Assembly Statistics
- Gene Expression Profile
- Advanced Data Analysis
- Blastnt or Blastnr
- Differentially Expressed Genes (DEGs)
- Gene Annotations & Ontology Analysis
Resequencing
- Standard Data Analysis
- Mapping Statistics
- Gene Expression Profile
- SNPs and lnDels calling by mapping to reference genome (Human, Mouse, Rat) - Novel Transcripts
- Alternative Spliced Transcripts
- Fusion Gene (Human, Mouse, Rat)
- Advanced Data Analysis
- Customized Analysis
- Differentially Expressed Genes (DEGs)
- Gene Ontology Analysis
Small RNA Sequencing
Particularly, small RNA molecules encoded from genomes are responsible for regulating the gene expression.
Macrogen's NGS technology is able to sequence and quantify all the small RNA families in a sample and profile the miRNA, siRNA, pi RNA and other non-coding RNAs.
Data Analysis*
Small RNA Sequencing
- Standard Data Analysis
- Expression Profiles of small RNA {piRNA, snoRNA, snRNA, Y RNA, vault RNA)
- Novel miRNA
- Advanced Data Analysis
- Target Prediction of Known and Novel miRNA
- Gene Set Analysis
- Differentially expressed mi RNA
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