5×Protein loading buffer (reduced)
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Categories : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
Brand : Servicebio
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Product Information
Product Name | Cat.No. | Spec. |
5× SDS-PAGE Loading Buffer (Reduced) | G2013-1ML | 1 mL |
G2013-100ML | 100 mL |
Description
The 5× SDS-PAGE Loading Buffer (Reduced), containing Tris-HCl buffer (63 mM, pH6.8), DTT (20 mM), glycerol (10%), SDS (2%) and bromophenol blue (0.0025%), is used for electrophoretic loading of reduced SDS-PAGE protein samples.
Storage and Handling Conditions
Product shipped at wet ice, stored at -20, valid for 12 months.
Component
Component | G2013-1ML | G2013-100ML |
5× SDS-PAGE Loading Buffer (Reduced) | 1 mL | 100 mL |
Protocol | 1 | |
Assay Protocol / Procedures
1. Mix each 4 μL protein sample with 1 μL 5× SDS-PAGE Loading Buffer (reduced sample).
2. Heat in a metal bath or boiling water bath for 3-5 minutes to fully denature the protein sample.
3. It can be added to the spotting well after cooling to room temperature.
Note
1. The product should be returned to room temperature before used.
2. Please wear lab coat and disposable gloves during operation.
For Research Use Only!
