PAS Staining Solution (for Microorganism staining)
Attribute:
SKU :
Option name 1
Categories : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
Brand : Servicebio
Share
Product Information
Product Name | Cat.No. | Spec. |
PAS Staining Kit | G1008-20ML | 3×20 ml |
G1008-100ML | 3×100 ml |
Product Introduction
This product PAS stain is used for glycogen PAS stain (Periodic Acid-Schiff stain), which is mainly used in tissue morphology to detect glycogen and polysaccharides in tissues. The main principle is to oxidize the hydroxyl groups on the two adjacent carbon atoms of sugar substances to aldehyde groups by the oxidizing agent periodic acid, and then react with the aldehyde groups to produce a purplish-red substance by using Schiff's reagent.
This product is a set, in which Solution A is Schiff Reagent, Solution B is 0.5% periodate solution, and Solution C is hematoxylin stain. After staining with PAS staining solution of this product, glycogen, neutral mucus material, cartilage matrix, epithelial basement membrane, plant fungi and cell wall in the tissues are purplish-red, and the nuclei of the cells are light blue.
Storage and Handling Conditions
The whole set of reagents can be stored at 2-8 away from light, of which PAS Solution B and PAS Solution C can be stored at room temperature, and the validity period is 12 months.
Component
Component Number | Component | G1008-20ML | G1008-100ML |
G1008-1 | PAS solution A | 20 mL | 100 mL |
G1008-2 | PAS solution B | 20 mL | 100 mL |
G1008-3 | PAS solution C | 20 mL | 100 mL |
Instruction Manual | 1 pc | ||
Preparation
Bring your own Hematoxylin differentiation solution (recommended G1039), Hematoxylin bluing solution (recommended G1040), xylene, gradient ethanol, anhydrous ethanol, neutral gum, etc.
Assay Protocol / Procedures
1. Dewaxing as followed:
Xylene I for 10 min;
Xylene II for 10 min;
100% ethanol I for 5 min;
100% ethanol II for 5 min;
90% ethanol for 5 min;
75% ethanol for 5 min;
Rinsing with tap water;
2. Stain sections with PAS solution B for 10-15 min, rinse three times with distilled water, each time about 10 s.
3. Stain with PAS solutino A (return to room temperature ahead of time for 25-30 min) in the dark, rinse for 5 min.
4. (Optional) Then stain it with PAS solution C for 30s, and rinse with tap water. Treat the slices with Hydrochloric acid solution and Ammonia, each step required washing with water. Then the sections were differentiated by hematoxylin differentiation solution for 3 s and washed with tap water; they were re-blued by hematoxylin bluing solution for 3 s and washed with tap water. This step is for cell nucleus staining.
5. Dehydrate as followed:
100% ethanol I for 5 min;
100% ethanol II for 5 min;
100% ethanol III for 5 min;
Xylene I for 5 min;
Xylene II for 5 min;
Finally seal with neutral gum.
Note
1. PAS solution B can be reused. The dye solution is transparent. If it is obviously yellow, contains impurities, or the staining of the tissue is too light, it needs to be replaced with a new dye solution.
2. PAS solution A needs to be stored at 4°C, take it out before use and return to room temperature before using. It is recommended to replace the dye solution with a new one when the color of the solution is obviously red.
3. Each set of staining solution (20 mL size) can be used to stain approximately 80 sections. Replace the stain with a new one when the tissue or cell coloring is significantly lighter or abnormal.
4. Please wear lab coat and disposable gloves during operation.
For Research Use Only!
