1× SWE Rapid High Resolution Running Buffer, 1 L
Attribute:
SKU : G2149-1L
Categories : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
Brand : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
1× SWE Rapid High Resolution Running Buffer (Ready to Use) | G2149-1L | 1 L |
Product Description
1× SWE Rapid High Resolution Running Buffer (Ready to Use) is optimal to Tris-Glycine electrophoresis buffer for SDS-PAGE gel electrophoresis in Tris-Glycine. Compared with the traditional Tris-glycine running buffer, this product can be used for electrophoresis without adjusting the voltage according to the separation gel and the stacking gel, and it can be used for the whole process of electrophoresis at a constant voltage of 200-250 V, it takes about 30 min to complete the electrophoresis, which saves time and is more effective for the separation of small molecules. It can separate the 10 kDa and the 15 kDa bands in the prestained protein marker and clearly visible (gel concentration above 8%) to achieve rapid and efficient separation of proteins.
Storage and Shipping Conditions
Ship and store at room temperature. Valid for 12 months.
Assay Protocol
1. Open the lid and pour it into the electrophoresis tank for use.
2. Selection of different protein gel concentrations for different protein molecular weights, refer to the table below:
SDS-PAGE Separation Gel Concentration | Optimum Separation Range (kDa) (SWE Rapid High Resolution Running Buffer) |
6% | |
8% | 10-250 |
10% | 5-150 |
12% | 3-100 |
15% | ＜60 |
3. Constant voltage electrophoresis, 200-250 V, refer to the following table for electrophoresis time:
Comparison Table of Electrophoresis Voltage and Electrophoresis Time | |
Voltage(V) | Estimated Electrophoresis Time(min) |
200 | 35 |
220 | 30 |
250 | 25 |
Note
1. It is recommended to use it as soon as possible after opening the lid.
2. This product is suitable for conventional Tris-glycine system SDS-PAGE gels, such as our products G2003, G2037, G2041, G2042, G2043, G2044, G2045, G2060, G2061, G2062, G2063, G2064.
3. This product may have crystal precipitation in the case of low ambient temperature, this is a normal phenomenon, can be heated moderately to help dissolve, to be completely dissolved and cooled to room temperature before use.
4. High voltage electrophoresis is easy to produce high heat. Please select the appropriate voltage for electrophoresis according to the ambient temperature.
5. This electrophoresis solution can be reused 2-3 times, more times reuse is not recommended.
6. For your safty and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
