Presto™ Plasmid DNA Concentration Kit, 100 preps/kit
Attribute:
SKU : PC1001
Categories : 2. Cell & Molecular Biology , DNA / RNA Extraction Kit , Geneaid (Taiwan) ,
Brand : Geneaid (Taiwan)
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Presto™ Plasmid DNA Concentration Kit (PC0250/251, PC0500/501, PC1000/1001)
DNA Extraction/Plasmid DNA PurificationThe Presto™ Plasmid DNA Concentration Kit offers a simplified replacement for time-consuming plasmid DNA precipitation procedures. The Presto™ Plasmid Concentrator will quickly and efficiently concentrate plasmid DNA in anion-exchange chromatographic DNA purification eluates collected from Geneaid Plasmid Midi Columns, Macherey-Nagel NucleoBond® AX, NucleoBond® Xtra Columns, QIAGEN® tip 500 Columns etc. Plasmid eluates are precipitated with isopropanol then passed through a Presto™ Plasmid Concentrator using a syringe. Once contaminants are removed using a proprietary Desalting Buffer, the concentrator is dried and plasmid DNA is eluted with a small volume (100-400 μl) of low salt Elution Buffer. The Presto™ Plasmid Concentrator eliminates DNA pellet loss and incomplete solubilization of precipitates to ensure highly concentrated, purified plasmid DNA. In only 10 minutes, the plasmid DNA can be used directly in a variety of sensitive downstream applications, such as transfection, sequencing, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun.
DOCUMENTS
COMPLETE PROTOCOL
DESCRIPTION
Specifications (Cat. # PC0250/251, PC0500/501, PC1000/1001)
- Concentrate plasmid DNA in only 10 minutes compared to 60 minutes using traditional plasmid DNA precipitation!
- Convenient: replaces traditional, time-consuming plasmid DNA precipitation procedures
- Binding Capacity: 600 µg of plasmid DNA
- Recovery: up to 90%
- Concentration: up to 3.2 µg/µl
- Plasmid Size: 1-20 kb
- Elution Volume: 100-400 µl
- Dead Volume: 25 µl
- Operation Time: 10 minutes (manual), 20 minutes/6 preps (vacuum)
- Storage: dry at room temperature (15-25°C)
APPLICATIONS
transfection, sequencing, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun
COMPONENTS
- Presto™ Plasmid Concentrator
- Desalting Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- 20 ml syringe
- 1 ml syringe
QUALITY CONTROL
The quality of the Presto™ Plasmid DNA Concentration Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system.
