SuperiorScript III cDNA Synthesis Kit,200units/μl

Product description

SuperiorScript III Reverse Transcriptase is genetically engineered version of M-MLV RT with reduced RNase H activity and increased thermostability, thus this enzyme can synthesize first-strand cDNA at elevated temperatures up to 55 ℃. SuperiorScript III Reverse Transcriptase shows improved thermostability and reduced RNase H activity than the competitor’s enzyme. This enzyme is capable of synthesizing first strand cDNA at 42℃~55℃ with high specificity and yield. Synthesis of longer products (12 kb or more) are possible than the previous versions.

Characteristics

- Produces excellent yields of amplifiable cDNA

- Can synthesize long cDNA targets, up to 12 kb, from small amounts of input RNA

- Able to reverse transcribe through RNA secondary structures

- Strict enzyme purity specifications

Applications

- cDNA cloning or cDNA library preparation

- Two step RT-PCR (end-point or real-time quantitative PCR)

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free

- Inhibitor-free

- Satisfactory yield and length of cDNA products

RNase H digestion

- RNase H can improve PCR sensitivity especially on the long template after synthesizing first-strand cDNA by eliminating RNA template on cDNA:RNA hybrid.  RNase H which is component of this kit can be properly used if necessary.

- The existence of RNase H while synthesizing the first-strand cDNA could decrease the yield of full-length cDNA.