GENEzol™ TriRNA Pure Kit w/DNase Set

RNA Extraction/Tri RNAThe GENEzol™ TriRNA Pure Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

DESCRIPTION
Advantages (Cat. # GZXD050, GZXD100, GZXD200)

• Purify total RNA in 15 minutes!
  
• A cost effective phenol, guanidine isothiocyanate solution plus spin column system
  
• No chlorophorm phase separation
  
• No isopropanol RNA precipitation
  
• No phenol carryover
  
• Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 10-50 mg of tissue, 1 x 109 bacterial cells, 20-50 mg of plant tissue
  
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
  
• Binding Capacity: 50 µg RNA from ≥ 25 µl DNase/RNase-free Water
  
• Spin Columns: glass fiber membrane optimized for total RNA extraction (certified RNase and DNase-free)
  
• Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, DNase I can be shipped at room temperature but should be stored at -20ºC for extended periods, other components should be stored at room temperature (15-25ºC)

APPLICATIONS
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

COMPONENTS

• GENEzol™ Reagent
  
• Pre-Wash Buffer
  
• DNase I
  
• DNase I Reaction Buffer
  
• Wash Buffer
  
• RNase-free Water
  
• RB Columns
  
• 2 ml Collection Tubes

QUALITY CONTROL
The GENEzol™ TriRNA Pure Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system.

GENEzol™ TriRNA Pure Kit Interactive Protocol

DOCUMENTS

GENEzol™ TriRNA Pure Kit

https://geneaid.com/Tri-RNA/GZXD