SYNONYMS
Lum, Ldc, SLRR2D, Lumican, Keratan sulfate proteoglycan lumican, KSPG lumican.
INTRODUCTION
Lumican also known as LUM is a part of the small leucine-rich proteoglycan (SLRP) family which comprises decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. Furthermore, we can see that in these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the main keratan sulfate proteoglycan of the cornea however LUM is also distributed in interstitial collagenous matrices throughout the body. Lumican regulates collagen fibril organization and circumferential growth, corneal transparency, epithelial cell migration and tissue repair.
DESCRIPTION
LUM Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 328 amino acids (19-338 a.a) and having a molecular mass of 37.5kDa (Migrates at 40-57kDa on SDS-PAGE under reducing conditions).
LUM is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
SOURCE
Sf9, Baculovirus cells.
PHYSICAL APPEARANCE
Sterile Filtered colorless solution.
FORMULATION
LUM protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 20% glycerol.
STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
PURITY
Greater than 95.0% as determined by SDS-PAGE.
AMINO ACID SEQUENCE
QYYDYDIPLF MYGQISPNCA PECNCPHSYP TAMYCDDLKL KSVPMVPPGI KYLYLRNNQI
DHIDEKAFEN VTDLQWLILD LLENSKIK GKVFSKLKQL KKLHINYNNL TESVGPLPKS
LQDLQLTNNK ISKLGSFDGL VNLTFIYLQH NQLKEDAVSA SLKGLKSLEY LDLSFNQMSK
LPAGLPTSLL TLYLDNNKIS NIPDEYFKRF TGLQYLRLSH NELADSGVPG NSFNISSLLE
LDLSYNKLKS IPTVNENLEN YYLEVNELEK FDVKSFCKIL GPLSYSKIKH LRLDGNPLTQ SSLPPDMYEC LRVANEITVN VEHHHHHH.
SAFETY DATA SHEET
SDS
USAGE
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
