Metagenome Sequencing
NGS is emerging as a powerful tool for profiling complex microbial communities. This new technology dramatically reduces both the time and cost of DNA sequencing, making it possible for a small laboratory to completely sequence the genome of their favorite bacterium.
For many bacterial species of interest, various strains have been sequenced. These reference strains are attractive subjects in genetic engineering, as derived strains can easily be compared at the genomic level against the parental strain using Macrogen's NGS techniques.
Amplicon Metagenomic Sequencing
Metagenomic sequencing enables efficient identification of microorganism diversity in a specific environment.
Sequencing Types & Platforms
- 165 rDNA sequencing using MiSeq system
- V3-V4 Regions
- Customized Regions
- Full-length 16s rRNA sequencing on PacBio RS II
- 185 rDNA/ ITS sequencing
Shotgun Metagenomic Sequencing
Shotgun metagenomic sequencing is a comprehensive sampling method of all the genes in all the organisms present in a given mixed sample. The method enables to evaluate the microbial diversity and detect the abundance of species under different conditions. For shotgun sequencing, DNA is purified and randomly sheared into smaller fragments before sequencing. Shotgun metagenomic sequencing provides information both about which organisms are present and what metabolic processes are possible in the community.
Data Analysis*
Amplicon Metagenome Sequencing
- Standard Data Analysis
- Community Diversity Analysis (OTU)
- Probiotics Analysis
- Advanced Data Analysis
- Phylogenetic Tree
- Hierarchical Taxonomy Graph
- Heatmap
- PCA biplot
Shotgun Metagenome Sequencing
- Standard Data Analysis
- Assembly
- Gene Prediction & Annotation
- Taxonomy Analysis
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